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splice donor sequence (sd) from the chimeric intron of pci-neo  (Promega)

 
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    Structured Review

    Promega splice donor sequence (sd) from the chimeric intron of pci-neo
    Splice Donor Sequence (Sd) From The Chimeric Intron Of Pci Neo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chimeric+intron+from+pci/rna+splicing+system/us11072803-182-59-60
    Average 90 stars, based on 1 article reviews
    splice donor sequence (sd) from the chimeric intron of pci-neo - by Bioz Stars, 2026-10
    90/100 stars

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    Construct:

    Article Title: Linear and circular expression elements
    Article Snippet: .. To construct the inventors' standard reporter plasmid, pCMViLUC, the luciferase-encoding LUC+ gene from pGL3-basic (Promega, Inc.) was inserted as an MluI/XbaI fragment into expression vector pCMV-5 (Andersson et al., 1989), and a chimeric intron from pCI (Promega, Inc.) was added as a SacI/EcoRI fragment. .. The complete 3.4 kb LEE.LUC was built by PCR®-amplifying the CMVi promoter, luciferase coding sequence, and hGH terminator from plasmid template pCMViLUC by standard protocols (Perkin-Elmer, Inc.).

    Article Title: Tolerance and chronic hepatitis C virus
    Article Snippet: .. The liver-specific plasmid, AAT-NS5a, was constructed by cloning, in order, four copies of the 154 bp human apolipoprotein E (ApoE) enhancer (Shachter et al., 1993, J. Lipid Res., 34:1699-1707), the human alpha-1 anti-trypsin (AAT) promoter (−350 to +50)(Long et al., 1984, Biochemistry, 23:4828-4837), the chimeric intron from pCI (Promega, Madison, Wis.), a polylinker, and the BGH poly A signal, between the AAV ITRs of pD10. .. Both the ApoE enhancer element and the AAT promoter were amplified from human genomic DNA (Promega).

    Plasmid Preparation:

    Article Title: Linear and circular expression elements
    Article Snippet: .. To construct the inventors' standard reporter plasmid, pCMViLUC, the luciferase-encoding LUC+ gene from pGL3-basic (Promega, Inc.) was inserted as an MluI/XbaI fragment into expression vector pCMV-5 (Andersson et al., 1989), and a chimeric intron from pCI (Promega, Inc.) was added as a SacI/EcoRI fragment. .. The complete 3.4 kb LEE.LUC was built by PCR®-amplifying the CMVi promoter, luciferase coding sequence, and hGH terminator from plasmid template pCMViLUC by standard protocols (Perkin-Elmer, Inc.).

    Article Title: Tolerance and chronic hepatitis C virus
    Article Snippet: .. The liver-specific plasmid, AAT-NS5a, was constructed by cloning, in order, four copies of the 154 bp human apolipoprotein E (ApoE) enhancer (Shachter et al., 1993, J. Lipid Res., 34:1699-1707), the human alpha-1 anti-trypsin (AAT) promoter (−350 to +50)(Long et al., 1984, Biochemistry, 23:4828-4837), the chimeric intron from pCI (Promega, Madison, Wis.), a polylinker, and the BGH poly A signal, between the AAV ITRs of pD10. .. Both the ApoE enhancer element and the AAT promoter were amplified from human genomic DNA (Promega).

    Luciferase:

    Article Title: Linear and circular expression elements
    Article Snippet: .. To construct the inventors' standard reporter plasmid, pCMViLUC, the luciferase-encoding LUC+ gene from pGL3-basic (Promega, Inc.) was inserted as an MluI/XbaI fragment into expression vector pCMV-5 (Andersson et al., 1989), and a chimeric intron from pCI (Promega, Inc.) was added as a SacI/EcoRI fragment. .. The complete 3.4 kb LEE.LUC was built by PCR®-amplifying the CMVi promoter, luciferase coding sequence, and hGH terminator from plasmid template pCMViLUC by standard protocols (Perkin-Elmer, Inc.).

    Expressing:

    Article Title: Linear and circular expression elements
    Article Snippet: .. To construct the inventors' standard reporter plasmid, pCMViLUC, the luciferase-encoding LUC+ gene from pGL3-basic (Promega, Inc.) was inserted as an MluI/XbaI fragment into expression vector pCMV-5 (Andersson et al., 1989), and a chimeric intron from pCI (Promega, Inc.) was added as a SacI/EcoRI fragment. .. The complete 3.4 kb LEE.LUC was built by PCR®-amplifying the CMVi promoter, luciferase coding sequence, and hGH terminator from plasmid template pCMViLUC by standard protocols (Perkin-Elmer, Inc.).

    Cloning:

    Article Title: Tolerance and chronic hepatitis C virus
    Article Snippet: .. The liver-specific plasmid, AAT-NS5a, was constructed by cloning, in order, four copies of the 154 bp human apolipoprotein E (ApoE) enhancer (Shachter et al., 1993, J. Lipid Res., 34:1699-1707), the human alpha-1 anti-trypsin (AAT) promoter (−350 to +50)(Long et al., 1984, Biochemistry, 23:4828-4837), the chimeric intron from pCI (Promega, Madison, Wis.), a polylinker, and the BGH poly A signal, between the AAV ITRs of pD10. .. Both the ApoE enhancer element and the AAT promoter were amplified from human genomic DNA (Promega).

    Bioprocessing:

    Article Title: Tolerance and chronic hepatitis C virus
    Article Snippet: .. The liver-specific plasmid, AAT-NS5a, was constructed by cloning, in order, four copies of the 154 bp human apolipoprotein E (ApoE) enhancer (Shachter et al., 1993, J. Lipid Res., 34:1699-1707), the human alpha-1 anti-trypsin (AAT) promoter (−350 to +50)(Long et al., 1984, Biochemistry, 23:4828-4837), the chimeric intron from pCI (Promega, Madison, Wis.), a polylinker, and the BGH poly A signal, between the AAV ITRs of pD10. .. Both the ApoE enhancer element and the AAT promoter were amplified from human genomic DNA (Promega).



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